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KMID : 1007520100190030717
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Food Science and Biotechnology
2010 Volume.19 No. 3 p.717 ~ p.724
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Cytotoxic Effects of 7-O-Butyl Naringenin on Human Breast Cancer MCF-7 Cells
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Park Jong-Hwa
Lee Jin-Woo
Paik Hyun-Dong
Cho Ssang-Goo
Nah Seung-Yeol
Park Yong-Sun
Han Ye-Sun
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Abstract
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The effect of 7-O-butyl naringenin (BN), a chemically synthesized derivative of naringenin, was tested on the proliferation of human breast cancer MCF-7 cells. BN inhibited the proliferation of MCF-7 cells in dosedependent manner (IC50: 67.5±2.1 μM), resulting in an increase in the sub-G1 phase cell population. BN induced the generation of intracellular reactive oxygen species (ROS), which were reduced by pretreatment with Nacetylcysteine (NAC). BN also increased the phosphorylation of stress-activated protein kinase/c-Jun NH4-terminal kinase 1/2 (SAPK/JNK1/2), c-Jun, and p38. However, the phosphorylation of extracellular-regulated kinase 1/2 (Erk1/2) was decreased in BN-treated cells. Pretreatment of cells with the specific inhibitors SP600125 and SB203580 diminished the BN-induced activation of SAPK/JNK1/2 and p38, respectively. These results indicate that the BN-induced cytotoxicity of MCF-7 cells is mediated by the generation of ROS as well as through the p38, SAPK/JNK1/2, and c-Jun activation signaling pathways. BN may therefore possess chemotherapeutic potential as an anti-proliferative agent.
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KEYWORD
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7-O-butyl naringenin, reactive oxygen species (ROS), stress-activated protein kinase/c-Jun NH4-terminal kinase 1/2 (SAPK/JNK1/2), p38, c-Jun
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